PDE8A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE8A
Enzyme
Human PDE8A
Synonym
cAMP-specific 3′,5′-cyclic phosphodiesterase 8A
Scientific Information
O60658 (http://www.uniprot.org/uniprot/O60658)
Assay Properties
Substrate
0.2 µM cAMP
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| PF-04671536 | 41.3 |
| TC-5005 | 19900 |
| Dipyridamole | 75800 |
| Trequinsin | 34400 |
| Milrinone | >100000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services.
Reference Compound IC50 for PDE8A
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