PDE8B Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE8B
Enzyme
Human PDE8B
Synonym
cAMP-specific 3′,5′-cyclic phosphodiesterase 8B
Scientific Information
O95263 (http://www.uniprot.org/uniprot/O95263)
Assay Properties
Substrate
0.2 µM cAMP
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| PF-04671536 | 1.2 |
| Zaprinast | 58100 |
| Dipyridamole | >100000 |
| Trequinsin | 5700 |
| Milrinone | >100000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services.
Reference Compound IC50 for PDE8B
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