PDE9A Phoshodiesterase Assay Service

PDE9A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#

PDE9A

Enzyme

Human PDE9A

Synonym

High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A

Substrate

0.2 µM cGMP

Assay Properties
Reaction

Nucleoside 3',5'-cyclic phosphate + H₂O = nucleoside 5'-phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
IBMX >100,000
Methoxymethyl-IBMX >100,000
Roliptam >100,000
Zaprinast 10,500
Scientific Information

O76083 (http://www.uniprot.org/uniprot/O76083)

Screening Location

Malvern, USA

Reference Compound IC50 for PDE9A

Reference Compound IC50 for PDE9A