PDE9A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE9A
Enzyme
Human PDE9A
Synonym
cGMP-specific 3′,5′-cyclic phosphodiesterase 9A
Scientific Information
O76083 (http://www.uniprot.org/uniprot/O76083)
Assay Properties
Substrate
0.2 µM cAMP
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Zaprinast | 19700 |
| Sildenafil | 27700 |
| Trequinsin | >100000 |
| Milrinone | >100000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services.
Reference Compound IC50 for PDE9A
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