PIK3CD/PIK3R1 NanoBRET Kinase Assay

PIK3CD/PIK3R1 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.

Target Overview



HGNC Symbol



p110D, P110DELTA, PI3K, PI3K-delta, p110-delta

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently co-expressing NanoLuc-PIK3CD Fusion Vector and PIK3R1 Expression Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-3 and then treated with reference compound PI-103 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.


Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
PI-103 3.197e-008

Screening Location

Malvern, PA, USA

Reference compound IC50 for PIK3CD/PIK3R1

Reference compound IC50 for PIK3CD/PIK3R1