PIP5K1B NanoBRET Kinase Assay

PIP5K1B NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target. 

Target

PIP5K1B

HGNC Symbol

PIP5K1B

Synonyms

MSS4, STM7

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-PIP5K1B Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-8 and then treated with reference compound CC1 Pan-Kinase Inhibitor for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
CC1 Pan-Kinase Inhibitor 1.476e-006
Screening Location

Malvern, USA

Reference compound IC50 for PIP5K1B