PRKCB NanoBRET Kinase Assay

PRKCB NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.

Target Overview
Target

PRKCB (beta)

HGNC Symbol

PRKCB

Synonyms

PRKCB1, PKCB, PRKCB2, MGC41878, PKC-beta, PKC-beta1

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties
Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-PRKCB Fusion Vector were seeded into the wells of 384-well plates. The transfected cells were stimulated with PMA, pre-treated with NanoBRET Tracer K-10, and then treated with reference compound Staurosporine for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.

Readout

Binding of compound to target

Reference Compound IC50s
Compounds IC50 (M)
Staurosporine 2.803e-009
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website NanoBRET Target Engagement Intracellular Kinase Assay Services

Reference compound IC50 for PRKCB

Reference compound IC50 for PRKCB

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