PXR

Nuclear receptor assay, cell-based luciferase reporter assay

 

Target

Pregnane X Receptor (PXR)

HGNC symbol

NR1I2

Synonyms

Steroid and xenobiotic sensing nuclear receptor (SXR)

Cell line

Human PXR Reporter Cell Line

Assay description:

- assay format: 96-well format

- construct: the PXR receptor contains the yeast GAL4 DNA binding sequence, which is functionally linked to a resident luciferase reporter gene (INDIGO biosciences)

- readout: receptor activity

 

reference compounds PXR assay

Human PXR Reporter cells were plated in 96-well plate and then stimulated with various concentrations of reference agonist Rifampicin for 24 hours. Luminescence signal was measured with an EnVision 2104 Multilabel Reader. PXR activation dose-response curve was plotted using GraphPad Prism program.