Nuclear receptor assay, cell-based luciferase reporter assay
Pregnane X Receptor (PXR)
Steroid and xenobiotic sensing nuclear receptor (SXR)
Human PXR Reporter Cell Line
- assay format: 96-well format
- construct: the PXR receptor contains the yeast GAL4 DNA binding sequence, which is functionally linked to a resident luciferase reporter gene (INDIGO biosciences)
- readout: receptor activity
Human PXR Reporter cells were plated in 96-well plate and then stimulated with various concentrations of reference agonist Rifampicin for 24 hours. Luminescence signal was measured with an EnVision 2104 Multilabel Reader. PXR activation dose-response curve was plotted using GraphPad Prism program.