Pregnane X Receptor Assay Service

PXR Nuclear Receptor Assay for compound screening in intact cells via luciferase reporter assay to quantify nuclear receptor activity.
Target Overview


Pregnane X Receptor (PXR)

HGNC symbol



Steroid and xenobiotic sensing nuclear receptor (SXR)

Cell line

Human PXR Reporter Cell Line

Assay description:

– assay format: 96-well format

– construct: the PXR receptor contains the yeast GAL4 DNA binding sequence, which is functionally linked to a resident luciferase reporter gene (INDIGO biosciences)

– readout: receptor activity


Assay Properties

Further information

More information can be found on our website Nuclear Receptor Assay Services.

reference compounds PXR assay

Human PXR Reporter cells were plated in 96-well plate and then stimulated with various concentrations of reference agonist Rifampicin for 24 hours. Luminescence signal was measured with an EnVision 2104 Multilabel Reader. PXR activation dose-response curve was plotted using GraphPad Prism program.