SENP1 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
SENP1
Enzyme
Human SENP1
Synonym
Sentrin-specific protease 1, Sentrin/SUMO-specific protease SENP1
Substrate
Fluorogenic substrate [SUMO1-AMC], 100 nM
Assay Properties
Reaction
Cleavage of SUMO1 for release of fluorescent AMC
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCHL1 I 2 | 240 |
| UCHL3 I | 132 |
Scientific Information
Q9P0U3 (http://www.uniprot.org/uniprot/Q9P0U3)
Screening Location
Malvern, USA
Reference Compound IC50 for SENP1
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