SENP1 Ubiquitin-Proteasome Pathway Assay Service

SENP1 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#

SENP1

Enzyme

Human SENP1

Synonym

Sentrin-specific protease 1, Sentrin/SUMO-specific protease SENP1

Substrate

Fluorogenic substrate [SUMO1-AMC], 100 nM

Assay Properties
Reaction

Cleavage of SUMO1 for release of fluorescent AMC

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (µM)
UCHL1 I 2 240
UCHL3 I 132
Scientific Information

Q9P0U3 (http://www.uniprot.org/uniprot/Q9P0U3)

Screening Location

Malvern, USA

Reference Compound IC50 for SENP1

Reference Compound IC50 for SENP1