SENP2 Ubiquitin-Proteasome Pathway Assay Service

SENP2 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#

SENP2

Enzyme

Human SENP2

Synonym

Sentrin-specific protease 2, Sentrin/SUMO-specific protease SENP2, SMT3-specific isopeptidase 2, Axam2, Smt3ip2

Substrate

Fluorogenic substrate [SUMO1-AMC], 300 nM

Assay Properties
Reaction

Cleavage of SUMO1 for release of fluorescent AMC

Readout

Enzymatic activity

Reference Compound IC50s

Compounds IC50 (µM)

UCHL3 I 2.8
Scientific Information

Q9HC62 (http://www.uniprot.org/uniprot/Q9HC62)

Screening Location

Malvern, USA

Reference compound IC50 for SENP2

Reference compound IC50 for SENP2