SGK2 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
HEK293 cells transiently expressing NanoLuc-SGK2 Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-9 and then treated with reference compound CC1 pan-Kinase inhibitor for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.
Binding of compound to target kinase
|CC1 pan-Kinase inhibitor||7.263e-007|
Malvern, PA, USA
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Reference Compound IC50 for SGK2