UCHL1 Ubiquitin-Proteasome Pathway Assay Service

UCHL1 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#

UCHL1

Enzyme

Human UCHL1

Synonym

Ubiquitin carboxyl-terminal hydrolase isozyme L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, UCH-L1)

Substrate

Fluorogenic substrate [Ubiquitin-AMC], 100 nM

Assay Properties
Reaction

Cleavage of ubiquitin for release of fluorescent AMC

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (µM)
Ub Aldehyde 17.8
UCHL1 I 2 16.7
UCHL3 I 88.3
Scientific Information

P09936 (http://www.uniprot.org/uniprot/P09936)

Screening Location

Malvern, USA

Reference Compound IC50 for UCHL1

Reference Compound IC50 for UCHL1