UCHL5 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
UCH-L5
Enzyme
Human UCH-L5
Synonym
UCH37, Ubiquitin thioesterase L5
Scientific Information
Q9Y5K5 (http://www.uniprot.org/uniprot/Q9Y5K5)
Assay Properties
Substrate
Fluorogenic substrate [Ubiquitin Rhodamine 110 Protein], 100 nM
Reaction
Ubiquitin Rhodamine 110 = Ubiquitin + Rhodamine 110
Rhodamine 110 fluorescence, kinetic measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCH-L3 Inhibitor (CAS # 30675-13-9) |
8.6 |
Screening Location
Malvern, PA, USA
Reference compound inhibition of UCHL5
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