USP10 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP10
Enzyme
Human USP10
Synonym
Ubiquitin carboxyl-terminal hydrolase 10, Deubiquitinating enzyme 10, Ubiquitin thiolesterase 10, Ubiquitin-specific-processing protease 10, KIAA0190, UBP10
Substrate
Fluorogenic substrate [Ubiquitin-AMC], 100 nM
Assay Properties
Reaction
Cleavage of ubiquitin for release of fluorescent AMC
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCHL1 I | 260 |
| UIPase I | >300 |
| UI1 | 170 |
Scientific Information
Q14694 (http://www.uniprot.org/uniprot/Q14694)
Screening Location
Malvern, USA
Reference Compound IC50 for USP10
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