USP10 Ubiquitin-Proteasome Pathway Assay Service

USP10 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#

USP10

Enzyme

Human USP10

Synonym

Ubiquitin carboxyl-terminal hydrolase 10, Deubiquitinating enzyme 10, Ubiquitin thiolesterase 10, Ubiquitin-specific-processing protease 10, KIAA0190, UBP10

Substrate

Fluorogenic substrate [Ubiquitin-AMC], 100 nM

Assay Properties
Reaction

Cleavage of ubiquitin for release of fluorescent AMC

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (µM)
UCHL1 I 260
UIPase I >300
UI1 170
Scientific Information

Q14694 (http://www.uniprot.org/uniprot/Q14694)

Screening Location

Malvern, USA

Reference Compound IC50 for USP10

Reference Compound IC50 for USP10