USP10 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP10
Enzyme
Human USP10
Synonym
Ubiquitin carboxyl-terminal hydrolase 10, Deubiquitinating enzyme 10, Ubiquitin thiolesterase 10, Ubiquitin-specific-processing protease 10, KIAA0190, UBP10
Scientific Information
Q14694 (http://www.uniprot.org/uniprot/Q14694)
Assay Properties
Substrate
Fluorogenic substrate [Ubiquitin Rhodamine 110 Protein], 100 nM
Reaction
Ubiquitin Rhodamine 110 = Ubiquitin + Rhodamine 110
Rhodamine 110 fluorescence, kinetic measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCH-L1 Inhibitor 1 | 12.6 |
| Wu-5 | 50.2 |
| Ubiquitin Aldehyde | 0.3 |
Screening Location
Malvern, PA, USA
Reference compound inhibition of USP10
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