USP13 Deubiquitination Assay Service

USP13 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.

Target Overview

CAT#

USP13

Enzyme

Human USP13

Synonym

Isopeptidase T-3, ISOT-3

Scientific Information

Q92995 (https://www.uniprot.org/uniprotkb/Q92995)

Assay Properties

Substrate

100 nM Ubiquitin Rhodamine 110 Protein

Reaction

Ubiquitin Rhodamine 110 = Ubiquitin + Rhodamine 110
Rhodamine 110 fluorescence, kinetic measurement

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (µM)
UCH-L1 Inhibitor 14.5
UCH-L1 Inhibitor 2 49.0
UCH-L3 Inhibitor 20.1
Spautin-1 >100

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Deubiquitination Assays (DUB assays)

Reference Compound IC50 for USP13

Reference Compound IC50 for USP13