USP14 Ubiquitin-Proteasome Pathway Assay Service

USP14 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#

USP14

Enzyme

Human USP14

Synonym

Ubiquitin carboxyl-terminal hydrolase 14, Deubiquitinating enzyme 14, Ubiquitin thiolesterase 14, Ubiquitin-specific-processing protease 14, TGT, UBP14

Substrate

Fluorogenic substrate [Ubiquitin-AMC], 100 nM

Assay Properties
Reaction

Cleavage of ubiquitin for release of fluorescent AMC

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (µM)
UCHL1 I 72.4
Ub Aldehyde 2.8
UCHL1 2 76.5
Scientific Information

P54578 (http://www.uniprot.org/uniprot/P54578)

Screening Location

Malvern, USA

Reference Compound IC50 for USP14

Reference Compound IC50 for USP14