USP2 Ubiquitin-Proteasome Pathway Assay Service

USP2 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#

USP2

Enzyme

Human USP2

Synonym

Ubiquitin carboxyl-terminal hydrolase 2, 41 kDa ubiquitin-specific protease, Deubiquitinating enzyme 2, Ubiquitin thiolesterase 2, Ubiquitin-specific-processing protease 2, UBP41, UBP2

Substrate

Fluorogenic substrate [Ubiquitin-AMC], 100 nM

Assay Properties
Reaction

Cleavage of ubiquitin for release of fluorescent AMC

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (µM)
UCHL1 I 23.2
UIPase I 165
UI1 >200
Scientific Information

O75604 (http://www.uniprot.org/uniprot/O75604)

Screening Location

Malvern, USA

Reference Compound IC50 for USP2

Reference Compound IC50 for USP2