USP30 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
USP30
Enzyme
Human USP30
Synonym
Ubiquitin carboxyl-terminal hydrolase 30
Scientific Information
Assay Properties
Substrate
100 nM Ubiquitin Rhodamine 110 Protein
Reaction
Ubiquitin Rhodamine 110 = Ubiquitin + Rhodamine 110
Rhodamine 110 fluorescence, kinetic measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| MF-094 | 0.012 |
| UCH-L1 Inhibitor | 13.5 |
| UCH-L1 Inhibitor 2 | >100 |
| UCH-L3 Inhibitor | >100 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Deubiquitination Assays (DUB assays)
Reference Compound IC50 for USP30
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