USP5 Ubiquitin-Proteasome Pathway Assay Service

USP5 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#

USP5

Enzyme

Human USP5

Synonym

Ubiquitin carboxyl-terminal hydrolase 5, Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thiolesterase 5, Ubiquitin-specific-processing protease 5, ISOT, UBP5

Substrate

Fluorogenic substrate [Ubiquitin-AMC], 100 nM

Assay Properties
Reaction

Cleavage of ubiquitin for release of fluorescent AMC

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (µM)
UCHL1 I 34.4
UIPase I 126
UI1 >32.8
Scientific Information

P45974 (http://www.uniprot.org/uniprot/P45974)

Screening Location

Malvern, USA

Reference Compound IC50 for USP5

Reference Compound IC50 for USP5