USP8 Ubiquitin-Proteasome Pathway Assay Service

USP8 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#

USP8

Enzyme

Human USP8

Synonym

Ubiquitin carboxyl-terminal hydrolase 8, Deubiquitinating enzyme 8, Ubiquitin isopeptidase Y, Ubiquitin thiolesterase 8, Ubiquitin-specific-processing protease 8, KIAA0055, UBPY, UBP8

Substrate

Fluorogenic substrate [Ubiquitin-AMC], 100 nM

Assay Properties
Reaction

Cleavage of ubiquitin for release of fluorescent AMC

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (µM)
UCHL1 I 8.3
UIPase I >200
UI1 72.9
Scientific Information

P40818 (http://www.uniprot.org/uniprot/P40818)

Screening Location

Malvern, USA

Reference Compound IC50 for USP8

Reference Compound IC50 for USP8