VHL Binding Assay Service

VHL binding assay for compound screening and profiling via probe displacement monitored through a change in fluorescence polarization

Target Overview
CAT#

VHL Direct

Enzyme

Human VHL/EloB/EloC complex

Probe

FAM-DEALAHypYIPMDDDFQLRSF

Scientific Information

P40337 (https://www.uniprot.org/uniprotkb/P40337/entry)

Assay Properties
Reaction

VHL + probe = FP signal

Readout

Change in FP signal upon probe displacement

Reference Compound IC50s
Compounds IC50 (nM)
MZ1 540.2
Screening Location

Malvern, PA, USA

Further information

More information can be found on our website Protein Degradation Assay – PROTAC Screening

Reference compound inhibition of probe binding to VHL