ALK Cellular Phosphorylation Assay Service

ALK Cellular Phosphorylation Assay (intracellular kinase activity assay) for compound screening and profiling in intact cells

Target Overview

Target Description

The anaplastic lymphoma receptor tyrosine kinase ALK, which is highly expressed in the neonatal brain, belongs to the Insulin receptor superfamily. The kinase is involved in the development and maintenance of the central and peripheral nervous system. In most anaplastic large-cell non-Hodgkin’s lymphomas developed from activated T lymphocytes, a (2;5) chromosomal translocation is found, which causes a fusion of the nucleophosmin gene NPM to the catalytic domain of ALK (NPM-ALK). This gene fusion results in a constitutive ligand-independent kinase activation.

HGNC Reference




Cell Line


Target Expression


Assay Properties

Assay Description

The human T cell lymphoma cell line Karpas-299 carries a (2;5) chromosomal translocation, which results in the expression of an ALK variant fused to nucleophosmin (NPM-ALK). This fusion results in constitutive, ligandindependent receptor tyrosine autophosphorylation of ALK, which can be reduced by adding ALK specific inhibitors like Crizotinib (see Fig. 1). In the cellular phosphorylation assay levels of phospho-ALK are quantified by Sandwich-ELISA technique.


Substrate phosphorylation as a readout of intracellular kinase activity via ELISA

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Cellular Phosphorylation Assay Services.

Reference compound IC50 for ALK

Reference compound IC50 for ALK

Crizotinib potently inhibits the autophosphorylation of ALK in the described cells with highly reproducible IC50 values. The graph shows the result of a representative experiment.