KRas Binding Kinetic Assay Service via SPR

KRAS-related Assays: Binding kinetics of agents to K-Ras are measured via surface plasmon resonance (SPR) technique

Target

K-Ras

HGNC

KRAS

Protein

K-Ras fragments aa 2-169 with a C-terminal biotinylation. They are likely in the GDP-bound state. Expressed in E. coli

Synonyms

C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2

Scientific information

K-Ras: P01116 (https://www.uniprot.org/uniprot/P01116)

Screening location

Malvern, PA, US

Reaction

Real-time peptide binding to K-Ras which is immobilized on a sensor chip

Binding kinetics of KRpep-2d to wild type K-Ras and mutants G12C, G12D, and G12V

Kras SPR WT G12D
Kras SPR G12C G12V
Kras SPR Table


The data was collected on a Biacore 8K using single-cycle kinetics. The data was fit using a 1:1 kinetic binding model. The peptide binds with the tightest affinity to K-Ras mutant G12D.