KRAS-related Assays: K-Ras binding assay service for compound screening and profiling using the thermal shift assay format to measure compound binding to the target
KRAS aa 2-169, N-terminal his tag. Expressed in E. coli.
KRAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2
KRas: P01116 (https://www.uniprot.org/uniprot/P01116)
Malvern, PA, US
Compound binding to K-Ras
Protein stability change due to ligand binding via thermal shift assay
The typical protein concentration in assay is 0.15 mg/ml
Variable, 25 µM is recommended
|G12C peak 1||4.7||3.3||0.5||0.25|
|G12C peak 2||12.5||16.5||no 2nd peak||no 2nd peak|
Note: 0.5°C is a typical noise observed in thermal shift assay which is not significant.
Reference compound testing in thermal shift assay with K-RAS wild type and mutants