Thermal Shift Assay Details
Example of Differential Scanning Fluorimetry of cGAS in the presence or absence of 10 µM PF-06928215.
The thermal denaturation of cGAS is detected via SYPRO Orange dye. The addition of the inhibitor PF-06928215 stabilizes the protein and increases the melting temperature from 45 to 53 degrees Celsius.
Differential Scanning Fluorimetry of BRD2-Tndm in the presence or absence of (+)- JQ1. The thermal denaturation of BRDT-Tndm is detected via SYPRO Orange dye. Addition of the BET Bromodomain inhibitor/ligand (+)- JQ1 stabilizes the protein folding and shifts the Tm from 41 to 44 degrees Celsius.
Instruments: BCFX384 (BioRad) real-time PCR detection system
Turnaround time: The study is typically completed within 2 weeks after receipt of materials.
Projects are performed on a first-come-first-serve basis.
Sample requirements: Protein purity of 85% or more is required. The protein amount is project dependent. Typical usage is between 1-3 ug per reaction.
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Screening location: The assay is performed in Malvern, PA, USA.