RON Cellular Phosphorylation Assay Service

RON Cellular Phosphorylation Assay (intracellular kinase activity assay) for compound screening and profiling in intact cells

Target Overview

Target Description

The receptor tyrosine kinase RON is a also known as MST1R (macrophage stimulating 1 receptor [also known as “c-met-related tyrosine kinase”]). The primary single chain precursor protein is post-translationally cleaved to produce alpha and beta subunits, which are disulfide linked to form the mature cell-surface receptor. Binding of the ligand MSP (macrophage-stimulating protein) activates receptor autophosphorylation. RON activity regulates cell survival, migration and differentiation of epithelial cells during wound healing and promotes migration and phagocytic activity of macrophages in the innate immune response.

HGNC Reference



CD136, CDw136, PTK8, RON

Cell Line


Target Expression


Assay Properties

Assay Description

The human breast cancer cell line T47D is known to express high levels of RON. Stimulation of these cells with its physiological ligand macrophage-stimulating protein (MSP) results in a robust receptor autophosphorylation. Compounds are preincubated before cell stimulation to allow thorough target binding. Stimulation conditions are optimized to determine dose-related inhibition of the phospho-RON signal, which is subsequently quantified by Sandwich-ELISA technique. Figure 1 shows data confirming the inhibitory activity of Crizotinib, a cognate RON inhibitor.


Substrate phosphorylation as a readout of intracellular kinase activity via ELISA

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Cellular Phosphorylation Assay Services.

Reference compound IC50 for RON

Reference compound IC50 for RON

Crizotinib is a potent inhibitor of the phospho-RON signal as observed in T47D breast cancer cells. The graph shows a representative result.