In Vivo Immunophenotyping via Flow Cytometry

Clinical data indicate that only some patients respond to treatment with immune-modulating drugs. Therefore, it is essential to consider the immune response during preclinical drug development. Flow cytometry is widely used for the analysis of immune cell populations in tumors as a means to describe the immune response.

Panel: A comprehensive analysis of numerous immune cell populations in one tumor is a challenge. We use established, ready-to-use staining panels that allow for the analysis of various immune cell populations. In addition, we can custom-tailor our existing panels or establish new antibody panels to match our customer's research needs.

Method: Primary tumor material is disrupted, erythrocytes removed, and up to 3x10⁶ obtained single cells/well are dispensed. The single cells are stained with marker-specific fluorescent antibodies and analyzed by flow cytometry.

Report: All data of the mouse study, including tumor growth and flow cytometry data, will be presented in a comprehensive report, custom-tailored to the client study, and written by a Ph.D.-level technical writer. The analysis enables you to draw conclusions directly from the study outcome and use the data for the filing of official documents.

Add-ons: Further immunophenotyping methods are available at Reaction Biology to investigate the cytokine & chemokine concentrations in the tumor via multiplex immunoassays, to localize tumor-infiltrating immune cells via IHC, or to test the immune cell function via in vitro assays.