The generation of cell lines stably expressing foreign proteins can serve many purposes such as labeling cells to visualize their location or insertion of a gene of interest.
Labeling of cell lines is necessary for live tracking of tumor cells in vivo or to perform proliferation studies via luciferase quantification. Based on extensive hands-on experience in genetic engineering of cells, Reaction Biology uses two approaches for genetic modifications of cell lines:
Standard transfection methods such as lipofection/nucleofection or Ca2PO4-transfection
Knock-out or knock-in of genes of interest via shRNA transfection
Lentiviral transduction that allows for highly efficient genetic manipulation, not only of cell lines but also of primary cells and non-dividing cells