Stable Cell Line Generation Service

The Cell Line Generation Service is used to create custom cell lines stably expressing foreign proteins can serve many purposes such as labeling cells to visualize their location or insertion of a gene of interest.

The generation of cell lines expressing labels such as luciferase is necessary for live tracking of tumor cells in vivo or to perform proliferation studies via luciferase quantification. Based on extensive hands-on experience in the genetic generation of stable cell lines, Reaction Biology uses two approaches for genetic modifications of cell lines:

  • Standard transfection methods such as lipofection/nucleofection or Ca2PO4-transfection
  • Knock-out or knock-in of genes of interest via shRNA transfection
  • Lentiviral transduction allows for highly efficient genetic manipulation, not only of cell lines but also of primary cells and non-dividing cells
Procedure

procedure to stably transfect cell lines for expression of genes

 

Deliverables
  • 2 million cells of newly generated cell lines (depending on licensing)
  • Data sheet including data of expression analysis