List of COVID-19 Protease Assays (8)
|ACE2 protease||angiotensin I converting enzyme 2||Data sheet|
|Cathepsin B protease||APP secretase, Cathepsin B1, CATB, CTSB, CPSB||Data sheet|
|Cathepsin L protease||CTSL, CTSL1||Data sheet|
|Furin protease||FUR, PACE, PCSK3||Data sheet|
|Mpro protease||3CLpro, main protease, 3cl protease||Data sheet|
|PLpro protease||NSP3, Papain-Like cysteine protease||Data sheet|
|TMPRSS2 protease||Transmembrane protease serine 2, PRSS10||Data sheet|
|TMPRSS6 protease||Transmembrane protease serine 6, matriptase 2||Data sheet|
See our full portfolio on our Protease Assay webpage.
SARS-CoV-2 Protease Assay Details
The papain-like protease (PLpro) is responsible for cleavage of the coronavirus polyprotein necessary for virus assembly. PLpro also suppresses the host innate immune through the reversal of ubiquitination and ISGylation events. We offer the investigation of the proteolytic activity of PLpro for deubiquitination and for proteolysis of a peptide.
The PLpro assay allows for screening compounds using a fluorogenic substrate, either Z-RLRGG-AMC or Ubiquitin-AMC. PLpro cleaves the substrate for the release of AMC and the resulting fluorescence can be quantified with a luminometer for readout of PLpro enzymatic activity.