Carboxylase Assay Services

Acetyl-CoA Carboxylase (ACC) is a biotin-dependent enzyme that catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA. ACC is a crucial metabolic enzyme and an attractive drug target. Reaction Biology provides compound screening against ACC by detecting the production of ADP.

  • Low and large scale screening as well as high-throughput screening options are available
  • Readout: enzymatic activity
  • Custom-tailored assay development possible
  • Carboxylases are produced in house and available for purchase. 

List of Targets

Target Synonyms Data
ACC1 Acetyl-CoA Carboxylase 1, ACACA Data sheet
ACC2 Acetyl-CoA Carboxylase 2, ACACB Data sheet

Assay Details

Assay Format

 

Assay principle for compound screening against carboxylase activity via quantification of ADP-glo

 

Acetyl-CoA carboxylase produces ADP when catalyzing the transformation of hydrogen carbonate and Acetyl-CoA to Malonyl-CoA. Drug candidates that inhibit the enzymatic activity of the carboxylase result in less ADP production which is measured with the ADP-Glo kit from Promega.

Assay Setup

Setups: Single-dose screening in duplicates or IC50 value determination with 5 or 10 concentrations. Other screening formats are available upon request.

Controls: No inhibitor (DMSO vehicle) control and for every assay, one target-specific control compound is tested in 10-dose IC50 format. 

Turnaround time: 10 business days for standard projects. Expedited scheduling and data delivery can be arranged prior to the commencement of the studies.

Report: The raw data, % enzyme activity and control compound IC50 values will be reported in Excel format for single-dose assays. For IC50 orders, raw data, IC50 values, and curve fitting will be delivered in Excel format. Assay conditions, target, and substrate information are available upon request. Requirements for this information should be noted prior to the commencement of the study.

Screening facility: This assay is performed at our screening facility in Malvern, PA, US.

Compound requirements: In brief, for a standard project, 20 µl of a 10 mM DMSO stock or solid material is needed. Less material is needed for large scale screening. Please refer to our FAQs for information regarding compound preparation and shipping.