NK Cell Killing Assays Available at Reaction Biology
Reaction Biology offers a versatile suite of immuno-oncology assay setups. Since every project is different, we enable a customized study layout with various options for immune cells, target cells, or readouts.
Please inquire about further customization requirements to tailor an assay specific to your research needs.
Options for immune cells
- PBMCs
- isolated NK cells
Options for target cells
- human or murine tumor cells
- tumor cells stably expressing tumor-associated antigens or luciferase
- 2D or 3D cell culture
Options for readout
- quantification of luciferase-expressing tumor cells
- flow cytometry
- live-cell imaging and high-content imaging
- Bliss factor determination of drug combination therapies
Readout options
- NK-mediated ADCC assay
- NK cell killing mediated by NK cell engager molecules
- further readout options are available upon request
Approach
In this study example, we explore the effect of the monoclonal anti-Her2 antibody Trastuzumab (Trz) on NK cell killing by co-cultivating PBMCs with tumor cells expressing the cognate antigen.
Setup
Luciferase-expressing tumor cells exhibiting Her2 are seeded in a 384 well plate. PBMCs at different E:T (effector : target cell) ratios are added on top of the tumor cells. After an incubation period of 16 hours in the presence of Trastuzumab, the luciferase activity is measured as a readout of tumor cell viability.
Study results
Trastuzumab (Trz)-induced immune killing of luciferase-labeled SKOV-3 tumor cells.
PBMCs from a healthy donor were incubated at different E:T ratios in the presence of various Trz concentrations with the Her2-expressing cell line SKOV-3-FireLuc. After 16-18h incubation, luciferase activity was measured, and the % viability was calculated using 1% Triton-treated cells as maximal cell death and untreated SKOV-3-FireLuc as maximal viability. The bar graph shows the mean and SD of % viability normalized to the untreated (no Trz) condition.