Cell-Based Assays

A variety of tumor cell-based assays for testing of therapeutic efficacy is available for the discovery of anti-cancer drugs. Most of our cellular assays are high-throughput compatible and can be customized according to your specific requirements.

Cell-based assays allow researchers to investigate the potency of novel drugs in more complex systems after primary screening in biochemical assays. Cellular assays allow compound screening at physiologic pH, ion, and co-factor concentrations allowing meaningful conclusions before commencing animal testing.

In addition to phenotypical assays, we offer cell-based assays of specific targets such as kinases, epigenetic proteins as well as GPCRs, ion channels, and nuclear receptors.

If you cannot find a suitable off-the-shelf cell assay for your research needs please get in touch to discuss the option of developing a custom-tailored cell-based assay with our scientists.

The ProLiFiler enables high-throughput analysis of the effects of test compounds on a panel of human tumor cell lines using the Cell Proliferation Assay.

Our Cell Proliferation & Viability Assays are 2D tumor cell-based assays for testing the therapeutic efficacy of anti-cancer drugs. We offer a variety of assay formats to investigate cell viability covering various cell death mechanisms.

The trans-well based invasion assay is used to analyze the therapeutic efficacy of test compounds on the capacity of tumor cells to degrade extracellular matrixes and transversing the basement membrane which are the steps necessary to invade new tissue for metastasis formation.

The Cell Migration Assay is used to investigate the initial phase of the metastasis process wherein tumor cells migrate away from the primary tumor site.

Anchorage-independent growth of cells is one of the hallmark characteristics of cellular transformation and uncontrolled cell growth. The Soft Agar Assay is a 3D assay allowing single cells to grow to cell colonies independently of a solid surface.

The 3D Tumor Spheroid Assay is used to analyze the therapeutic efficacy of test compounds on three-dimensional cell clones either with mono-spheroids of only tumor cells or with co-spheroids of tumor and stroma cells.

Generation of stably engineered cells can be performed via the insertion of genes of interest or labels such as luciferase or GFP.

The Cellular Angiogenesis Assay is a spheroid-based assay suitable for the investigation of early events of angiogenesis – the sprouting of new blood vessels from endothelial tissue.

Reaction Biology offers a variety of readout options for cell-based oncology assays to gain an additional layer of information and allow assay setups tailored to our customers specific research needs.