qPCR Service

Real-time quantitative PCR (qPCR; RT-PCR) service at Reaction Biology provides custom-tailored gene expression analysis and copy number determination including high-quality DNA and RNA isolation. Relative and absolute quantification approaches enable the detection of quantitative differences in mRNA expression of targets, PD markers, or predictive biomarkers. Our qPCR platform is set up to support drug discovery projects performed with cellular and animal models at Reaction Biology, as well as investigation of customer samples sent to our lab. 

Our qPCR services have numerous applications in the drug discovery process, such as:

  • To test for target gene expression after knock-down studies or for identification of suitable tumor cell lines for drug efficacy testing
  • To screen biomarkers predicting a drug’s response in cellular or animal models.
  • To determine pharmacodynamic parameters of drugs that affect the expression of specific genes for the determination of time- or dose-dependency and mode of action analysis. 
  • Genotyping for verification of stratification markers

Reaction Biology offers complete in-house service comprising sample generation from our cellular and animal models through isolation of RNA and qPCR service.

Our scientists are available for consultation to meet your research needs with custom-designed project layouts. Our QuantStudio 6 Pro Real-Time PCR System from Applied Biosystems allows mRNA quantification, multiplex assays, and high-throughput projects. Please reach out today to discuss how we can help you best.

Quality data with a quality qPCR setup

QPCRs are performed with the state-of-the-art QuantStudio 6 Pro Real-Time PCR System (Applied Biosystems) for reliable and reproducible gene expression studies.  

All qPCR services are carried out in our qPCR lab with dedicated work areas for DNA and RNA extraction and handling.

Additional qPCR Service Information

qPCR Workflow

qPCR Workflow

Sample options

Sample preparation protocols are established for cultured cells and tissues harvested from mice, including tumor material.

Further sample options: 3D-cell culture, whole blood, PBMCs, fixed-formalin paraffin embedded (FFPE) samples, and cell-free samples such as cell culture supernatants, plasma to analyze cell-free, or circulating free DNA.

Quality Management

The sample preparation and qPCR analysis will be performed in our dedicated qPCR laboratory, with state-of-the-art equipment and specific work areas for each step.

Furthermore, we use verified protocols and standard procedures to achieve a high standard and minimize the risks of cross-contamination. During all steps, we only use high-quality kits for reliable and reproducible data generation. 

An integrated QC algorithm of the QuantStudio 6 software guarantees optimal performance of the Real-Time PCR reaction.

QC of RNA and DNA samples is performed spectrophotometrically with the UV5 Nano spectrophotometer from Mettler Toledo.

Addition of ROX reference dye and uracil-DNA glycosylase (UDG) to the PCR master mix enable the normalization of the intensity of the reporter fluorochrome and prevent false positives arising from carryover contaminations.