Biophysical assays enable understanding of the drug-target interaction on a molecular level. As a CRO we offer our clients primary and secondary biophysical screening options for the determination of the residence time, mechanism of action, and kinetics, all of which are crucial for the therapeutic action of a drug. Biophysical screening is thus essential for the selection of analytes with the most promising properties for the next stage of drug discovery.
Our team of biophysical assay experts, together with an array of cutting-edge instrumentation, is available for hit identification, hit-to-lead, and lead characterization activities tailored to the needs of your research project.
Reaction Biology offers off-the-shelf biophysical assays for high-throughput screening, custom assay development, and optimization as well as integrated projects in close interaction with our team of biophysical assay experts.
Thermal shift assays measure the thermal stability of a target protein and the increase in protein melting temperature upon the binding of a stabilizing agent. It is useful for identifying ligands, buffer conditions and cofactors affecting protein stability.
Four biophysical assays are offered at Reaction Biology CRO
Surface Plasmon Resonance (SPR)
Isothermal Titration Calorimetry (ITC)
Microscale Thermophoresis (MST)
Thermal Shift Assay (TSA)
Optical measurement that detects molecular mass
changes of the target upon analyte binding
Measures the heat released or absorbed during
analyte binding to a target
Measures the changes in the motion of target molecules
along microscopic temperature gradients upon analyte
Monitors the shift in melting temperature of a target upon