Protein Production and Assay Development

Reaction Biology can assist you with producing proteins and assays for various target classes such as kinases, epigenetics, PARPs, DUB, HSP, protease, phosphatase, and many more. Our expertise comprises producing proteins and multimeric protein complexes for expression in E. coli and insect cells. Our outstanding assay development teams enable drug testing in custom-tailored biochemical, cellular, biophysical, and electrophysiologic assay formats.

Our services comprise:

Small or large scale custom-tailored protein production
Assay development and optimization/validation
Enzyme testing, substrate testing, and activator/inhibitor testing

Our scientists have created more than 2200 assays over the last 20 years, including the successful completion of challenging projects. Please reach out to the business development manager in your area today to discuss how we can assist your protein production and assay development needs.

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Protein Production

Expression systems	Sf9, SFX and Sf21 insect cells E.coli
Tags	GST HIS GST-HIS HALO-HIS SUMO-HIS TXN-HIS Streptavidin Nus Flag Additional tags are available
Purification	Affinity chromatography Ion exchange Hydrophobic Size exclusion
Modification	Activation by autophosphorylation Activation by an upstream kinase Removal of tag Co-expression with co-factors Mutations Biotinylation Fluorescent labeling
QC options	SDS PAGE Bradford quantification Mass Spectrometry Enzymatic activity assay

Assay Development

Radiometric detection including:

³H
³³P
³²P

Fluorescence-based detection methods such as:

AlphaLISA
AlphaScreen
Fluorescence intensity
Fluorescence polarization
FRET
TR-FRET

Luminescence-based assays such as:

Bioluminescence, such as ADP-Glo and ATP bioluminescence-based assays
Chemiluminescence, such as horseradish peroxidase (HRP) and alkaline phosphatase (AP) based assays

Absorbance and colorimetric based assays

For biophysical detections we offer:

For cell-based detections we offer:

NanoBRET technology
Calcium mobilization
Second messenger detection
Enzymatic activity via substrate detection by using ELISA or Western blotting or Glo-based assays
TR-FRET-based competitive immunoassay
Electrophysiologic readouts via patch clamp technique
Gene expression quantification via luciferase reporter assays

Non-human Targets

Reaction Biology offers customized projects on non-human targets that may be necessary for testing the inhibitory activity of compounds on kinases in animal models such as mouse, dog or rat.

Please inquire to receive a list of available assays for screening of non-human targets.

Protein Production and Assay Development

Non-human Targets

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