Protein Production and Assay Development

Reaction Biology can assist you with the production of proteins and assays for a variety of target classes such as kinases, epigenetics, PARPs, DUB, HSP, protease, phosphatase, and many more.

Our services comprise:

  • Small or large scale custom-tailored protein production
  • Assay development and optimization/validation
  • Enzyme testing, substrate testing, and activator/inhibitor testing

Our scientists have created more than 1000 assays over the last 20 years including the successful completion of challenging projects. Please reach out to the business development manager in your area today to discuss how we can assist your protein production and assay development needs.

Protein Production
Expression systems
  • Sf9, SFX and Sf21 insect cells
  • E.coli
Tags
  • GST
  • HIS
  • GST-HIS
  • HALO-HIS
  • SUMO-HIS
  • TXN-HIS
  • Streptavidin
  • Nus
  • Flag
  • Additional tags are available
Purification
  • Affinity chromatography
  • Ion exchange
  • Hydrophobic
  • Size exclusion
Modification
  • Activation by autophosphorylation
  • Activation by an upstream kinase
  • Removal of tag
  • Co-expression with co-factors
  • Mutations
  • Biotinylation
  • Fluorescent labeling
QC options
  • SDS PAGE
  • Bradford quantification
  • Mass Spectrometry
  • Enzymatic activity assay

 

Assay Development

Radiometric detection including:

  • 3H
  • 33P
  • 32P


Fluorescence-based detection methods such as:

  • AlphaLISA
  • AlphaScreen
  • Fluorescence intensity
  • Fluorescence polarization
  • FRET
  • TR-FRET


Luminescence-based assays such as:

  • Bioluminescence, such as ADP-Glo and ATP bioluminescence-based assays
  • Chemiluminescence, such as horseradish peroxidase (HRP) and alkaline phosphatase (AP) based assays


Absorbance and colorimetric based assays


For biophysical detections we offer:

 

For cell-based detections we offer:

  • NanoBRET technology
  • Calcium mobilization
  • Second messenger detection
  • Enzymatic activity via substrate detection by using ELISA or Western blotting or Glo-based assays
  • TR-FRET-based competitive immunoassay
  • Electrophysiologic readouts via patch clamp technique
  • Gene expression quantification via luciferase reporter assays

 

 

Non-human Targets

Reaction Biology offers customized projects on non-human targets that may be necessary for testing the inhibitory activity of compounds on kinases in animal models such as mouse, dog or rat.

Please inquire to receive a list of available assays for screening of non-human targets.