Protein Production and Assay Development

Reaction Biology can assist you with the production of proteins and assays for a variety of target classes such as kinases, epigenetics, PARPs, DUB, HSP, protease, phosphatase, and many more.

Our services comprise:

Small or large scale custom-tailored protein production
Assay development and optimization/validation
Enzyme testing, substrate testing, and activator/inhibitor testing

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Protein Production

Expression systems	Sf9 and Sf21 insect cells E.coli
Available tags	GST HIS GST-HIS HALO-HIS SUMO-HIS TXN-HIS Additional tags are available
Modification	Activation by autophosphorylation Activation by an upstream kinase Removal of tag Co-expression with co-factors Mutations

Assay Development

Both biochemical and biophysical based assays are typically used for detecting enzyme activities, protein-protein interactions, and protein-small molecule bindings etc.

For biochemical detections we offer:

Radiometric detection including:

³H
³³P
³²P

Fluorescence-based detection methods such as:

Alpha-Lisa
Alpha-Screen
FI
FP
FRET
TR-FRET

Luminescence-based assays such as:

Bioluminescence, such as ADP-Glo, ATP-bioluminescence based assays
Chemiluminescence, such as horseradish peroxidase (HRP) and alkaline phosphatase (AP) based assays

Absorbance and colorimetric based assays

For biophysical detections:

SPR
MST
ITC
Thermal shift

Protein Production and Assay Development

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