Protein Production and Assay Development

Reaction Biology can assist you with the production of proteins and assays for a variety of target classes such as kinases, epigenetics, PARPs, DUB, HSP, protease, phosphatase, and many more.

Our services comprise:

  • Small or large scale custom-tailored protein production
  • Assay development and optimization/validation
  • Enzyme testing, substrate testing, and activator/inhibitor testing
Protein Production
Expression systems
  • Sf9 and Sf21 insect cells
  • E.coli
Available tags
  • GST
  • HIS
  • GST-HIS
  • HALO-HIS
  • SUMO-HIS
  • TXN-HIS
  • Additional tags are available
Modification
  • Activation by autophosphorylation
  • Activation by an upstream kinase
  • Removal of tag
  • Co-expression with co-factors
  • Mutations

 

Assay Development

Both biochemical and biophysical based assays are typically used for detecting enzyme activities, protein-protein interactions, and protein-small molecule bindings etc.    

For biochemical detections we offer:

Radiometric detection including:

  • 3H
  • 33P
  • 32P


Fluorescence-based detection methods such as:

  • Alpha-Lisa
  • Alpha-Screen
  • FI
  • FP
  • FRET
  • TR-FRET


Luminescence-based assays such as:

  • Bioluminescence, such as ADP-Glo, ATP-bioluminescence based assays
  • Chemiluminescence, such as horseradish peroxidase (HRP) and alkaline phosphatase (AP) based assays


Absorbance and colorimetric based assays


For biophysical detections: 

  • SPR
  • MST
  • ITC
  • Thermal shift