Protein Production and Assay Development

Reaction Biology can assist you with the production of proteins and assays for a variety of target classes such as kinases, epigenetics, PARPs, DUB, HSP, protease, phosphatase, and many more.

Our services comprise:

Small or large scale custom-tailored protein production
Assay development and optimization/validation
Enzyme testing, substrate testing, and activator/inhibitor testing

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Protein Production

Expression systems	Sf9 and Sf21 insect cells E.coli
Available tags	GST HIS GST-HIS HALO-HIS SUMO-HIS TXN-HIS Additional tags are available
Modification	Activation by autophosphorylation Activation by an upstream kinase Removal of tag Co-expression with co-factors Mutations

Assay Development

Radiometric detection including:

³H
³³P
³²P

Fluorescence-based detection methods such as:

AlphaLISA
AlphaScreen
Fluorescence intensity
Fluorescence polarization
FRET
TR-FRET

Luminescence-based assays such as:

Bioluminescence, such as ADP-Glo and ATP bioluminescence-based assays
Chemiluminescence, such as horseradish peroxidase (HRP) and alkaline phosphatase (AP) based assays

Absorbance and colorimetric based assays

For biophysical detections we offer:

For cell-based detections we offer:

NanoBRET technology
Calcium mobilization
Second messenger detection
Enzymatic activity via substrate detection by using ELISA or Western blotting or Glo-based assays
TR-FRET-based competitive immunoassay
Electrophysiologic readouts via patch clamp technique
Gene expression quantification via luciferase reporter assays

Protein Production and Assay Development

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