Protein Production and Assay Development

Radiometric detection including:

• ³H
• ³³P
• ³²P

Fluorescence-based detection methods such as:

• AlphaLISA
• AlphaScreen
• Fluorescence intensity
• Fluorescence polarization
• FRET
• TR-FRET

Luminescence-based assays such as:

• Bioluminescence, such as ADP-Glo and ATP bioluminescence-based assays
• Chemiluminescence, such as horseradish peroxidase (HRP) and alkaline phosphatase (AP) based assays

Absorbance and colorimetric based assays

For biophysical detections we offer:

• Surface Plasmon Resonance
• Microscale Thermophoresis
• Isothermal Titration Calorimetry
• Thermal shift assay

For cell-based detections we offer:

• NanoBRET technology
• Calcium mobilization
• Second messenger detection
• Enzymatic activity via substrate detection by using ELISA or Western blotting or Glo-based assays
• TR-FRET-based competitive immunoassay
• Electrophysiologic readouts via patch clamp technique
• Gene expression quantification via luciferase reporter assays