Protein Production and Assay Development
Reaction Biology can assist you with producing proteins and assays for various target classes such as kinases, epigenetics, PARPs, DUB, HSP, protease, phosphatase, and many more. Our expertise comprises producing proteins and multimeric protein complexes for expression in E. coli and insect cells. Our outstanding assay development teams enable drug testing in custom-tailored biochemical, cellular, biophysical, and electrophysiologic assay formats.
Our services comprise:
- Small or large scale custom-tailored protein production
- Assay development and optimization/validation
- Enzyme testing, substrate testing, and activator/inhibitor testing
Our scientists have created more than 2200 assays over the last 20 years, including the successful completion of challenging projects. Please reach out to the business development manager in your area today to discuss how we can assist your protein production and assay development needs.
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Radiometric detection including:
- 3H
- 33P
- 32P
Fluorescence-based detection methods such as:
- AlphaLISA
- AlphaScreen
- Fluorescence intensity
- Fluorescence polarization
- FRET
- TR-FRET
Luminescence-based assays such as:
- Bioluminescence, such as ADP-Glo and ATP bioluminescence-based assays
- Chemiluminescence, such as horseradish peroxidase (HRP) and alkaline phosphatase (AP) based assays
Absorbance and colorimetric based assays
For biophysical detections we offer:
- Surface Plasmon Resonance
- Microscale Thermophoresis
- Isothermal Titration Calorimetry
- Thermal shift assay
For cell-based detections we offer:
- NanoBRET technology
- Calcium mobilization
- Second messenger detection
- Enzymatic activity via substrate detection by using ELISA or Western blotting or Glo-based assays
- TR-FRET-based competitive immunoassay
- Electrophysiologic readouts via patch clamp technique
- Gene expression quantification via luciferase reporter assays
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