Sample Processing
Primary tumor tissue is disrupted and erythrocytes removed; in general 3×10⁶ single cells are stained
Immunophenotyping by Flow Cytometry
Characterize tumor-infiltrating immune populations with high-parameter spectral analysis
Quantify immune cell frequencies, activation states, and functional markers in tumor tissue using validated multi-color panels optimized for syngeneic and humanized mouse models.
Available Markers and Panels
Custom-tailored panels can be established upon request to detect the immune cell population of your interest.
| Marker | Panels |
|---|---|
| Arginase 1 | Not in any panel |
| B220 | All-in-One, Neuro-oncology |
| Calreticulin | Not in any panel |
| CCR4 (CD194) | Not in any panel |
| CCR7 | Not in any panel |
| CCR7 (CD197) | Not in any panel |
| CD107a | Not in any panel |
| CD115 | Not in any panel |
| CD11b | Spectral Analyzer, All-in-One, Neuro-oncology |
| CD11c | Spectral Analyzer, All-in-One, Neuro-oncology |
| CD135 | Not in any panel |
| CD14 | Not in any panel |
| CD16 | Not in any panel |
| CD163 | Not in any panel |
| CD19 | Spectral Analyzer |
| CD20 | Not in any panel |
| CD206 | Spectral Analyzer, All-in-One, Neuro-oncology |
| CD209a | Not in any panel |
| CD24 | Not in any panel |
| CD3 | Neuro-oncology |
| CD34 | Not in any panel |
| CD39 | Not in any panel |
| CD3e | All-in-One |
| CD4 | Spectral Analyzer, All-in-One |
| CD40 | Spectral Analyzer |
| CD44 | Spectral Analyzer |
| CD45 | Spectral Analyzer, All-in-One, Neuro-oncology |
| CD45.2 | Not in any panel |
| CD48 | Not in any panel |
| CD49b | Spectral Analyzer, All-in-One |
| CD5 | Not in any panel |
| CD62L | Spectral Analyzer |
| CD64 | Not in any panel |
| CD68 | Neuro-oncology |
| CD69 | Spectral Analyzer |
| CD73 | Not in any panel |
| CD80 | Spectral Analyzer |
| CD86 | Spectral Analyzer, Neuro-oncology |
| CD8a | All-in-One |
| CD8b | Spectral Analyzer |
| CGIQNSVSA (hCEA, Dextramer, H-2db) | Not in any panel |
| c-Kit (CD117) | Not in any panel |
| CTLA-4 (CD152) | Not in any panel |
| Eomes | Not in any panel |
| F4/80 | Spectral Analyzer, All-in-One, Neuro-oncology |
| FoxP3 | Spectral Analyzer, All-in-One |
| Gal-1 | Not in any panel |
| Gal-3 | Not in any panel |
| Granzyme B | Not in any panel |
| HSP70 | Not in any panel |
| ICOS (CD278) | Not in any panel |
| IFNg | Not in any panel |
| IL-2R α (CD25) | All-in-One |
| IL-2Rbeta (CD122) | Not in any panel |
| IL-7Rα (CD127) | Not in any panel |
| iNOS | Not in any panel |
| Integrin alpha E (CD103) | Not in any panel |
| Ki-67 | Spectral Analyzer |
| KLRG1 | Not in any panel |
| LAG3 (CD223) | Spectral Analyzer |
| Ly-6A/E (Sca-1) | Not in any panel |
| Ly6C | Spectral Analyzer, All-in-One, Neuro-oncology |
| Ly6G | Spectral Analyzer, All-in-One, Neuro-oncology |
| Ly-6G/Ly-6C (Gr-1) | Not in any panel |
| mALCAM | Not in any panel |
| MHC Class II (I-A/I-E) | Spectral Analyzer, All-in-One, Neuro-oncology |
| MHC-I (H2-Kd) | Not in any panel |
| NK-1.1 | Not in any panel |
| NKG2 A/B/C | Not in any panel |
| NKG2AB6 | Not in any panel |
| NKp46 (CD335) | Spectral Analyzer, All-in-One |
| Nrp-1 (CD304) | Not in any panel |
| PD-1 (CD279) | Spectral Analyzer |
| PD-L1 (CD274) | Spectral Analyzer |
| Siglec H | Not in any panel |
| SIINFEKL (Dextramer, H-2kb) | Not in any panel |
| SIINFEKL (Pentamer, H-2kb) | Not in any panel |
| SIRPa (CD172a) | Not in any panel |
| SLAM (CD150) | Not in any panel |
| TCR gd | Not in any panel |
| TCR β chain | Spectral Analyzer |
| TER-119/Erythroid Cells | Not in any panel |
| Thy1.2 (CD90.2) | Not in any panel |
| Tim3 (CD366) | Spectral Analyzer |
| TNF a | Not in any panel |
| XCR1 | Not in any panel |
| TMEM119 | Neuro-oncology |
| P2RY12 | Neuro-oncology |
| CX3CR1 | Neuro-oncology |
| IBA1 | Neuro-oncology |
| CD49d | Neuro-oncology |
