Biomarker Discovery at Reaction Biology
Biomarker | Method |
---|---|
Changes in protein levels | Multiplex MSD, Flow cytometry |
Gene expression analysis | qPCR, Biomarker Analysis Tool |
Cell death mechanism | ROS generation, mitochondrial membrane potential, Annexin V staining, cell morphology |
Immunophenotype | Flow cytometry, Multiplex Immunoassays, IHC and TMA, Immune cell activity |
Pharmacodynamic markers | AlphaLISA, ELISA, or Western blotting |
Phenotypic changes | MoA Finder, IncuCyte live cell imaging, High-content imaging (coming soon) |
ELISA ... Enzyme-Linked Immunosorbent Assay
IHC........ Immunohistochemistry
MSD...... Multiplex Immunoassays via Meso Scale Discovery
qPCR..... Real-time quantitative PCR
TMA....... Tissue microarrays