Biomarker Discovery

Reaction Biology offers a suite of assays to support the discovery of biomarkers to identify and quantify the effects of drug treatment on cellular or animal models.

Goals of biomarker research:

  • Ensure that your compound acts on-target during the entire drug discovery process starting at hit identification.
  • Understanding the mode of action of the compound.
  • Finding a pharmacodynamic marker to monitor the efficacy of your compound and enabling dosing experiments in animal models.
  • Determine the immune-modulating activity of immunotherapeutics

Biomarker Discovery at Reaction Biology

Biomarker Method
Changes in protein levels Multiplex MSD, Flow cytometry
Gene expression analysis qPCR, Biomarker Analysis Tool
Cell death mechanism ROS generation, mitochondrial membrane potential, Annexin V staining, cell morphology
Immunophenotype Flow cytometry, Multiplex Immunoassays, IHC and TMA, Immune cell activity
Pharmacodynamic markers AlphaLISA, ELISA, or Western blotting
Phenotypic changes MoA Finder, IncuCyte live cell imaging, High-content imaging (coming soon)

ELISA ... Enzyme-Linked Immunosorbent Assay
IHC........ Immunohistochemistry
MSD...... Multiplex Immunoassays via Meso Scale Discovery
qPCR..... Real-time quantitative PCR
TMA....... Tissue microarrays

In addition to our in vitro assays, biomarkers can be studied in mice for PK/PD modeling in our state-of-the-art animal facility to determine dosing and your drug candidate’s ADME parameters.

View our suite of cutting-edge instrumentation enabling accurate and reproducible biomarker screening.

 

Genetic Biomarkers

Protein Biomarkers

Phenotypic Biomarkers

Immunophenotyping