Immunophenotyping

Reaction Biology offers three methods for immunophenotyping which is crucial for investigation of immunotherapeutics. The following options are available at Reaction Biology:

  • Flow cytometry is a perfect method to investigate the frequency of immune cells in tumor tissue and evaluate the immune-modulating effects of drugs. Find more information here.
  • Multiplexing immune markers including cytokines and chemokines can be performed by the highly sensitive mesoscale discovery (MSD) platform. Find more information here.
  • At the animal study endpoint, tumor tissue can be isolated and snap-frozen or embedded for histology or immunohistochemical investigation. A TMA can be assembled for streamlined processing and staining of several dozen tumor samples on one slide.
  • Tissue cores of tumors of all of our syngeneic tumor models are available in a tissue microarray (TMA). Slices of the TMA can be sent for staining to the client or the tissues can be stained with the marker of choice from us. Read more here.
    We provide images of our tumor samples upon request stained with H&E, anti-CD3, or anti-CD11b antibodies. Reach out to get insight into our tumor models.
Immunohistochemistry & TMA Immune cell localization via IHC Multiplex MSD Immune signatures via cytokine/chemokine quantification Flow Cytometry Quantification of Immune cell frequencies

Reaction Biology offers flow cytometry analysis for investigating the frequency of tumor-infiltrating immune cells, multiplex assays for testing the cytokine environment, and immunohistology to visualize the location of immune cells in tumor tissue. 

Talk to our Business Development Manager team today to discuss your immunophenotyping needs.