|Flow cytometer Instrument||Laser||Marker||Comments|
|BD LSR Fortessa||4||17|
|Guava easyCyte 8HT||2||8|
|Sony SP6800ZE Spectral Analyzer||3||20||full spectrum analyzer uses spectral unmixing instead of conventional compensation|
|Cell sorter Instrument||Laser||Marker||Comments|
|BD FACS Aria Fusion||4||18||Sort 4 cell populations simultaneously|
|BD FACS Aria III||5||21||Sort 4 cell populations simultaneously|
|Beckman Coulter MoFlo Astrios EQ||4||21||Sort 6 cell populations simultaneously|
Established staining panels
|CD4+ T cells|
|CD8+ T cells|
|M1 / M2 Macrophages|
|Markers:||Memory Status:||Activation Markers:|
|CD4+ T cells||CD62L||PD-1|
|CD8+ T cells||CD44||Ki67|
|CD4+ T cells||Granzyme B|
|CD8+ T cells||IFN-gamma|
|Treg cells||CD107a (lamp 1)|
Clinical data indicate that only a part of patients responds to treatment with immune-modulating drugs. It has hence been recognized as essential to consider the immune response already during preclinical drug development. Flow cytometry is widely used for the analysis of immune cell populations in tumors.
Panel: A comprehensive analysis of numerous immune cell populations in one tumor is a challenge. We use established, ready-to-use staining panels that allow for the analysis of various immune cell populations. In addition, we can custom-tailor our existing panels or establish new antibody panels to match our customer's research needs.
Method: Primary tumor material is disrupted, erythrocytes removed, and up to 3x106 obtained single cells/well are dispensed. The single cells are stained with marker-specific fluorescent antibodies and analyzed by flow cytometry.
Report: All data of the mouse study, including tumor growth and flow cytometry data, will be presented in a comprehensive report, custom-tailored to the client study, and written by a Ph.D.-level technical writer. The analysis enables you to draw conclusions directly from the study outcome and use the data for the filing of official documents.
Add-on cytokine quantification: We can perform cytokine and chemokine quantification with our MESO Quickplex SQ120 assay platform. Please inquire for more information.