Humanized Xenograft Models
Humanized xenograft models recapitulate the interplay between the tumor and the immune system occurring in patients by engrafting human tumor bearing murine hosts with human peripheral blood mononuclear cells (PBMC), T cells or NK cells.
These models enable rapid testing of human targeted immunotherapies and provide insights into a drug mechanisms of action, kinetics, toxicity, and potency.
Our team of immunology experts have developed a range of different humanized xenograft mouse models in which different components of the human immune system have been reconstituted to allow for the testing of efficacy and potency of your immuno-oncology drug candidate.
What we offer:
- A fully humanized platform to test human specific immunomodulators
- A simplified approach to humanization compared to humanized PDX
- A team of expert available to assist on study designs and data analysis
- T cell or NK cell reconstituted human tumor bearing models to evaluate your agent in vivo
- A diverse panel of human tumors-derived xenografts including AML and breast cancer
Reach out today to discover how our platform can help you advance your human immunotherapy research.
Humanized Xenograft Models Available at Reaction Biology
Set up:
To engineer the humanized MOLM-13 acute myeloid leukemia model, PBMCs were isolated from buffy coats from four human blood donors. T cells were isolated from PBMC fractions of each donor, and purity was determined using flow cytometry (Figure 2). NOG and NOD-SCID mouse strains were used for the study. On days -3 and -2, mice were pretreated with cyclophosphamide. On day 0, MOLM-13_Luc+ tumor cells were injected intravenously into all mice. Isolated T cells from donors 1 & 2 were injected intravenously into NOG mice on day 8. Isolated T cells from donors 3 & 4 were injected in NOD-SCID mice on day 15.
Goal:
Humanized MOLM-13 acute myeloid leukemia model is suitable for the investigation of bi-specific engagers using isolated T or NK cells from PBMCs of whole blood donors.
Results:
Figure 1: Tumor growth of the MOLM13_Luc cells was monitored using an IVIS®Lumina III bioluminescence imaging system (PerkinElmer).
Addition of T cells did not influence the exponential MOLM-13_Luc tumor growth in NOG mice and only slightly retarded the growth in NOD-SCID mice.
T-Cell Purity Check
Figure 2: T cells are obtained with a high purity by negative selection from buffy coat derived PBMCs.
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